Nodulation of Legumes

All legume plants can fix nitrogen in the right conditions. Nodules are the swollen, rounded lumps that are formed on the roots of legumes when there is an effective symbiosis in the rootzone with nitrogen-fixing rhizobia. Unless legumes are forming nodules, they are unlikely to be fixing much nitrogen.

The nodulation of legumes score is monitored & benchmarked in the Soilmentor Regen Platform.

N.B. The exact number of nodules you want for a high scoring root depends on the legume. E.g. You want to look for 50 or more nodules per plant for vetch, field pea, faba beans and lentils, and 10-30 per plant for chickpea for a score of 2.

Best to test in autumn and/or spring. Avoid carrying-out this test after heavy rain or when very dry.

What to record

  • Score
    • 0 = None. VERY POOR.
    • 0.5 = Very few small nodules high on roots. POOR. 
    • 1 = Some small nodules high up on the roots. MODERATE.
    • 1.5 = Nodules across the root profile, that are pink when cut open. GOOD. 
    • 2 = Many nodules across the root profile, pink or red in colour when cut. VERY GOOD.
  • Photos


  • Spade
  • Bucket & water (for washing the soil from roots)

How to do the test

1. Locate your chosen sample site – you can use the GPS mapping feature on the app to do this if you’ve recorded the sample locations previously.

2. Dig out a small section of soil, no wider than your spade or do with other spade tests (20cm x 20cm).

3. Gently break or wash away soil from roots of legumes.

4. Observe if there are any nodules on the roots. If so, select four of the largest nodules to cut to check the colour.

5. Record a score on the scale above in the Soilmentor app.

Watch the video below to see the nodulation of legumes method in action!